Biochemistry Kits
PREPARATION OF MITOCHONDRIA KIT (FROM PLANTS)
The kit describes the practical isolation of Mitochondria from plant material and demonstration of activity of isolated mitochondria.
CAT. NO. BTK 130901
Contents
Isolation Medium
Resuspension Medium
Activity Demonstration Solution
User Manual
PREPARATION OF CHLOROPLAST KIT (FROM PLANTS)
The kit describes the practical handling and interpretation of isolation of chloroplast from plant source and of activity of chloroplast by Hill’s reaction.
CAT. NO. BTK 130902
Contents
Isolation Medium
Resuspension Medium
Activity Demonstration Solution
User Manual
NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS KIT
The kit describes the practical handling of native PAGE technique for the separation of various types of protein from the crude cell protein extract and detection of an enzyme protein from this mixture by activity staining on gels.
CAT. NO. BTK 130903
Contents
Enzyme Protein Extract
Protein Molecular Weight Marker Lane
Acrylamide : Bisacrylamide Solution
Separating and Stacking Buffer
Electrophoresis Buffer
Protein Loading Dye & Staining Dye
Activity Staining Dye
Ammonium Persulphate
User Manual
SDS POLYACRYLAMIDE GEL ELECTROPHORESIS KIT
The kit describes the practical handling of SDS PAGE technique
for the separation of protein and determination of their
molecular weight.
CAT. NO. BTK 130904
Contents
Protein Sample
Protein Molecular Weight Marker Lane
Acrylamide : Bisacrylamide Solution
SDS-Separating & SDS-Stacking Buffer
SDS-Electrophoresis Buffer
Protein Loading Dye & Staining Dye
Ammonium Persulphate
User Manual
TWO DIMENSIONAL PAGE KIT
Two dimensional polyacrylamide gels developed by O’Farrell employs separation of denatured proteins according to two different parameters, Isoelectric point and molecular weight. The kit enables practical working with this gel system.
CAT. NO. BTK 130905
Contents
Acrylamide : Bisacrylamide Solution
Anode & Cathode Electrode Buffer
Sample Overlay Buffer
Electrophoresis Buffer
Stacking and Separation Buffer
Protein Staining Solution
User Manual
STARCH GEL ELECTROPHORESIS KIT
Starch gels exhibit molecular sieving effects. Separation of proteins on starch gel is not only on the basis of differences in charge, but also on differences in molecular size and shape. The kit enables practical working with starch gels.
CAT. NO. BTK 130906
Contents
Protein Sample
Protein Marker Lane
Starch
Protein Staining & Destaining Solution
Gel Buffer
Electrophoresis Buffer
User Manual
ACETIC ACID UREA PAGE KIT
In an Acetic Acid Urea Polyacrylamide Gel Electrophoresis, both the molecular size and charge act as the basis of protein separation. The kit enables practical working with acetic acid urea polyacrylamide gels.
CAT. NO. BTK 130907
Contents
Protein Sample
Protein Marker Lane
Acrylamide : Bisacrylamide Solution
Electrophoresis Buffer
Denaturation and Native Sample Buffer
Urea and Glacial Acetic Acid
Ammonium Persulphate
Protein Staining Solution
Protein Destaining Solution
User Manual
PROTEIN SILVER STAINING KIT
The kit describes the practical handling of most sensitive staining method available for staining of proteins from Polyacrylamide Gels with silver staining.
CAT. NO. BTK 130908
Contents
Fixing Solution
Destaining Solution
Silver Nitrate Solution
Developing Solution
PAGE Reagents
User Manual
PROTEIN ESTIMATION KIT
The kit describes practical performance and comparison of two widely used techniques for colourimetric estimation of protein. The techniques are Folin-Ciocalteau and Bradford.
CAT. NO. BTK 130909
Contents
Standard Protein
Sample Protein
Folin-Ciocalteau Reagent
Alkaline Soldium Carbonate Solution
CuS04 – Na K Tartarate Solution
Bradford’s Reagent
User Manual
PREPARATION OF LYSOSOME BODIES KIT
The kit describes the practical handling and interpretation of isolation of lysosome bodies from mammalian source and demonstration of activity of isolated lysosomal bodies.
CAT. NO. BTK 130910
Contents
Isolation Medium
Resuspension Medium
Activity Demonstration Solution
User Manual
GEL FILTRATION CHROMATOGRAPHY KIT
Gel filtration technique is used to separate proteins based on differences in their molecular size or to remove low-molecularweight contaminants such as salts, amino-acids, and peptides etc. The kit enables user for preparation of gel column, standardization of column and separation of protein mixture
CAT. NO. BTK 130911
Contents
Protein Sample
Protein Molecular Weight Markers
Gel Matrix
Gel Buffer
User Manual
ION-EXCHANGE CHROMATOGRAPHY KIT
Ion-exchange chromatography separates proteins based on molecular charge. Protein mixtures are applied to oppositely charged, chromatographic matrix and the various proteins are bound by reversible, electrostatic interactions. The kit enables the user practical understanding of Ion-Exchange chromatography technique.
CAT. NO. BTK 130912
Contents
Protein Sample
Elution Buffer
Wash Buffer
Gel Matrix
User Manual
AFFINITY GEL CHROMATOGRAPHY KIT
In affinity chromatography, gel matrix has affinity for the specified protein. The kit enables user practical understanding of the technique.
CAT. NO. BTK 130913
Contents
Crude Protein Sample
Protein Standard
Gel Matrix (Concanavalin A Agarose)
Sodium Acetate Buffer
Elution Buffer
Solution I, II and III
ABTS Solution
User Manual
AMINO-ACID PAPER ELECTROPHORESIS KIT
The paper electrophoresis of amino-acid separates amino-acid on the basis of charge. The kit enables user to understand the basis of amino-acid separation and identification.
CAT. NO. BTK 130914
Contents
Standard Amino-Acid Solution
Test Amino-Acids
Whatman Filter Paper
Electrophoresis Buffer
Amino-Acid Detection Reagent
User Manual
SERUM PROTEIN PAPER ELECTROPHORESIS KIT
The paper electrophorosis of serum protiens is one of the most important techniques in diagnoistic research. The kit enables user to seperate serum proteins n paper using technique of electrophorosis.
CAT. NO. BTK 130915
Contents
Standard Protein Marker
Electrophoresis Buffer
Staining Solution
Cellulose Acetate Paper Strips
Standard Fractionation Chart
User Manual
HAEMOGLOBIN PAPER ELECTROPHORESIS KIT
The paper electrophoresis of haemoglobin is one of the most widely used and most important techniques in diagnostic research for the detection of Haemoglobinopathies (Haemoglobin associated disorders). The kit enables user to separate abnormal haemoglobin on paper using technique of electrophoresis.
CAT. NO. BTK 130916
Contents
Lysis Buffer
Electrophoresis Buffer
Cellulose Acetate Paper Strips
Elution Buffer
Detection Reagent
Standard Fractionation Chart
User Manual
AMINO-ACID PAPER CHROMATOGRAPHY KIT
The paper chromatography separates amino acids. The Rf values of the sample amino-acid is calculated and compared with that of the standard amino-acids. The kit enables user to understand the basics of amino-acid separation and their identification.
CAT. NO. BTK 130917
Contents
Standard Amino-Acid Solution
Test Amino-Acids
Whatman Paper
Reservoir Buffer
Amino-Acid Detection Reagent
Glass Capillaries
Amino-Acid Rf-Reference Chart
User Manual
CARBOHYDRATE PAPER CHROMATOGRAPHY KIT
The paper chromatography separates carbohydrates. The Rf values of the sample carbohydrate is calculated and compared with that of the standard carbohydrate. The kit enables user to understand the basics of carbohydrate separation and their identification.
CAT. NO. BTK 130918
Contents
Standard Sugar Solution
Test Sugars
Whatman Paper
Reservoir Buffer
Sugar Detection Reagent
User Manual
WESTERN BLOTTING KIT
The kit describes the practical handling and interpretation of fractionation of protein by electrophoresis and then transfer of the separated patterns of proteins on solid support like nylon membrane.
CAT. NO. BTK 130919
Contents
Prtoein Sample
Molecular Weight Marker Lane
SDS-PAGE Contents
Nylon Memebrane
Denaturation Solution
Neutralization Solution
20X and 2X SSC
Whatman paper, Paper Towels, Glass
Plate, Sponge, Plastic Wrap
User Manual
COLORIMETRIC ESTIMATION OF SUGARS KIT
The kit describes practical performance and comparison of two widely used techniques for colourimetric estimation of sugar. The techniques are Phenol-Sulphuric Acid and DNSA method.
CAT. NO. BTK 130920
Contents
Standard Sugars
Test Sugars
Phenol Solution & Sulphuric Acid
DNSA Reagent
User Manual
CELL IMMOBILIZATION & DETECTION OF IMMOBILIZED CELL ACTIVITY KIT
The kit enables user to immobilize whole yeast cells in calcium alginate. The kit also provides detection system to study the activity of the immobilized cells i.e. production of alcohol.
CAT. NO. BTK 130921
Contents
Sodium Alginate
Calcium Chloride Solution
Cell Activity Detection System
Universal Yeast Medium
User Manual
SUGAR SPOT TEST DETECTION KIT
The kit enables qualitative detection of carbohydrates by simple detection reagent.
CAT. NO. BTK 130922
Contents
Standard Sugars
Sample Sugars
Molisch’s Reagent
Iodine Reagent
Fehling’s Reagent
Benedict’s Reagent
Barfoed’s Reagent
Bial’s Reagent
User Manual
PROTEIN SPOT TEST DETECTION KIT
The kit enables qualitative detection of proteins by simple detection reagent.
CAT. NO. BTK 130923
Contents
Standard Protein Samples
Biuret reaction
Ninhydrin Reagent
Sakaguchi Reagent
Hopkins-Cole Reagent
Millon’s Reagent
User Manual
ENZYME ACTIVITY SPOT TEST DETECTION KIT (FROM MICROBIAL SOURCE)
The kit enables qualitative detection of proteins by simple detection reagent.
CAT. NO. BTK 130924
Contents
Microbial Cultures
Amylase Detection Reagent
Cellulase Detection Reagent
Peroxidase & Catalase Detection Reagent
Pectinase & Xylanase Detection Reagent
Protease Detection Reagent
Nutrient Media and Agar
User Manual
ENZYME KINETICS KIT
The most important factors which affects the enzyme activity are enzyme concentration, substrate concentration, pH of reaction and temperature of reaction. The kit enables user to understand the practical implication of enzyme activity and factors affecting its rate of reaction.
CAT. NO. BTK 130925
Contents
Enzyme
Enzyme Substrate
Reaction Buffer
Chromogen Detection Solution
User Manual
COLORIMETRIC ESTIMATION OF AMINO-ACIDS KIT
The kit describes practical performance of widely used technique for colorimetric estimation of amino-acid.
CAT. NO. BTK 130926
Contents
Standard Amino-Acid Solution
Test Amino-Acid Solution
Detection Reagent
User Manual
DETERMINATION OF IODINE, SAPONIFICATION AND PEROXIDE VALUE KIT
Saponification is the process by which the fatty acids in the glycerides of the oil are hydrolysed by an alkali. Peroxide value is a measure of the peroxides contained in the oil. The iodine is a measure of the degree of unsaturation in oil.
CAT. NO. BTK 130927
Contents
Sodium thiosulphate solution
Starch solution
Potassium iodide solution
Solvent mixtures
Hydrochloric Acid & Alcoholic KOH
Phenolphthalein Indicator
User Manual
COLORIMETRIC ESTIMATION OF PHENOLIC COMPOUNDS KIT
Total phenolic compounds can be colorimetrically estimated with the Folin-Ciocalteau Reagent. The kit enables user to prepare a standard graph for various concentration of standard phenol and then estimation of unknown concentration of phenol.
CAT. NO. BTK 130928
Contents
Standard Phenolic solution
Test Phenolic compound
Ethanol solution
Folin-Ciocalteau Reagent
Sodium carbonate solution
User Manual
DIALYSIS KIT
Dialysis is one of the most commonly used techniques to separate sample components based on selective diffusion across a porous membrane. The kit enables the user to perform and then interpret the results obtained by separation and analysis of low molecular weight components from high molecular weight components.
CAT. NO. BTK 130929
Contents
Protein Sample
1M Phosphate Buffer (pH 7)
Bradford Dye Concentrate
Dialysis Tubing
User Manual
SERUM PROTEIN AGAROSE GEL ELECTROPHORESIS KIT
Serum protein electrophoresis is generally performed to identify patients who have paraproteins. The unique pattern of bands obtained by electrophoretic separation on agarose gels plays an important role in diagnostic purpose. The kit enables the user to perform the experiment and interpret the results obtained by comparing the pattern of bands obtained for healthy and diseased ones.
CAT. NO. BTK 130930
Contents
Electrophoresis Buffer
Staining Solution
Decolorizer
Protein Loading Dye
Agarose
User Manual
TOTAL PROTEIN EXTRACTION KIT (FROM FUNGI)
The kit enables user to perform fast and easy extraction of total proteins rom fungal pellets without the need for sonication or precipitation.
CAT. NO. BTK 130931
Contents
Fungal culture
Lysis Solution
Acrylamide : Bis Solution
Separating Buffer
Stacking Buffer
Ammonium Persulphate
Protein Loading Dye
Electrophoresis Buffer
Protein Staining Dye
Protein Loading Dye
Electrophoresis Buffer
Protein Staining Dye
User Manual
TOTAL PROTEIN EXTRACTION KIT (FROM MAMMALIAN CELLS)
The kit enables user to perform fast and easy extraction of total proteins from mammalian cells or tissues without the need for sonication or precipitation.
CAT. NO. BTK 130932
Contents
Acrylamide : Bis Solution
Lysis Solution
Separating Buffer
Stacking Buffer
Ammonium Persulphate
Protein Loading Dye
Electrophoresis Buffer
Protein Staining Dye
User Manual
TOTAL PROTEIN EXTRACTION KIT (FROM PLANT CELLS)
The kit enables user to perform fast and easy extraction of total proteins from plant cells without the need for sonication or precipitation.
CAT. NO. BTK 130933
Contents
Acrylamide : Bis Solution
Lysis Solution
Separating Buffer
Stacking Buffer
Ammonium Persulphate
Protein Loading Dye
Electrophoresis Buffer
Protein Staining Dye
User Manual
TOTAL PROTEIN EXTRACTION KIT (FROM BACTERIA)
The kit enables user to perform fast and easy extraction of total proteins from bacterial cells without the need for sonication or precipitation.
CAT. NO. BTK 130934
Contents
Bacterial culture
Lysis Solution
Acrylamide : Bis Solution
Separating Buffer
Stacking Buffer
Ammonium Persulphate
Protein Loading Dye
Electrophoresis Buffer
Protein Staining Dye
User Manual
SGOT KIT
The in vitro qualitative determination of activity of enzymes AST / GOT in human serum / plasma for diagnosis of myocardial infarction can be carried out using the SGOT Kit.
CAT. NO. BTK 130935
Contents
Reaction Component I
Reaction Component II
User Manual
SGPT KIT
The in vitro qualitative determination of activity of enzymes ALT / GPT in human serum / plasma for evaluating liver function can be carried out using the SGPT Kit.
CAT. NO. BTK 130936
Contents
Reaction Component I
Reaction Component II
User Manual
BLOOD GLUCOSE ESTIMATION KIT (BY GOD-POD METHOD)
Enzymatic methods for estimation of glucose are considered to be very simple, cheap, and accurate. The GOD-POD method is widely used to detect abnormalities if any in the blood plasma or serum.
CAT. NO. BTK 130937
Contents
Glucose Standard
Test Glucose
GOD-POD Reagent
User Manual
SEPARATION OF LIPOPROTEINS BY AGAROSE GEL ELECTROPHORESIS KIT
The relative concentrations of lipoproteins in serum may provide useful information in the differentiation of diseases of various body systems. Electrophoresis produces important banding pattern to identify any fluctuation in these concentration.
CAT. NO. BTK 130938
Contents
Electrophoresis Buffer
Staining Solution
Decolorizer
Fixative Solution
Protein Loading Dye
Agarose
User Manual
2D PAPER CHROMATOGRAPHY KIT (FOR AMINO-ACIDS)
When dealing with the mixtures of unknown amino-acids in the samples, Two-Dimensional Chromatography becomes very useful to separate all the amino-acid on the single phase. The kit provides a mixture of known and unknown amino-acids with detailed procedure to understand the principle and application of Two-Dimensional Paper Chromatography.
CAT. NO. BTK 130939
Contents
Solvent System I
Solvent System II
Staining Reagent
Test Samples
Standard Samples
Whatman Paper Sheets
User Manual
DETERMINATION OF FREE AND VOLATILE FATTY ACIDS KIT
The kit enables estimation of free fatty acids present in oils along with the triglycerides and estimation of the volatile fatty acids which are water soluble.
CAT. NO. BTK 130940
Contents
Potassium hydroxide solution
Phenolphthalein solution
Neutral solvent
Dilute sulphuric acid solution
Ferric chloride solution
Magnesium sulphate
User Manual
PREPARATION OF DENSITY GRADIENT COLUMNS AND CELL FRACTIONATION KIT
The kit enables user to form sucrose gradient column and fractionize the complex extract of plant or animal tissue.
CAT. NO. BTK 130941
Contents
Sucrose Concentration Solution
User Manual
THIN LAYER CHROMATOGRAPHY KIT (FOR SUGARS)
Thin layer chromatography method is best suitable for the separation of low molecular weight compounds like sugars, amino-acids and lipids. The kit enables user to identify unknown sugars from the mixture by the aid of ready-to-use thin layer chromatography plates and detection system.
CAT. NO. BTK 130942
Contents
Solvent System
Staining Reagent
Test Samples
Standard Samples
Thin Layer Chromatography Plates
User Manual
THIN LAYER CHROMATOGRAPHY KIT (FOR LIPIDS)
Thin layer chromatography method is best suitable for the separation of low molecular weight compounds like sugars, amino-acids and lipids. The kit enables user to identify unknown lipids from the mixture on the basis of their polarity by the aid of ready-to-use thin layer chromatography plates and detection system.
CAT. NO. BTK 130943
Contents
Solvent System
Staining Reagent
Test Samples
Standard Samples
Thin Layer Chromatography Plates
User Manual
SEPARATION OF LDH ISOENZYMES BY PAGE KIT
Serum lactic dehydrogenase (LDH) is an ideal enzyme for the analysis of isozymes, and is also a model system for electrophoretic analysis. Since these isoenzymes are usually associated with characteristic tissue or organ sources, their relative concentrations in serum may provide useful information in the differentiation of diseases of various body systems. The kit contains reagents to carry out the separation of LDH Isoenzymes by PAGE from test serum.
CAT. NO. BTK 130944
Contents
Acrylamide : Bis Solution
Separating Buffer
Ammonium Persulphate
Protein Loading Dye
Electrophoresis Buffer
Protein Staining Dye
Activity Staining Dye
TEMED
Acetic Acid
User Manual
DETECTION OF BETA-LACTAMASE ACTIVITY KIT
The emergence of resistance to beta-lactam antibiotics has caused considerable interest in rapid, reliable techniques for recognition of resistant isolates. The kit enables user to recognize the bacterial cultures producing beta-lactamase by four different techniques.
CAT. NO. BTK 130945
Contents
Bacterial Culture
Acido Detection Strips
Iodo Detection Strips
Chromogen
Nutrient Media and Agar
User Manual
MITOCHONDRIA PREPARATION KIT (FROM MAMMALIAN TISSUES)
The kit enables user to prepare protoplasts mitochondria from mammalian cells for numerous molecular and cellular studies.
CAT. NO. BTK 130946
Contents
Isolation Medium
Resuspension Medium
Activity Demonstration Solution
User Manual
PROTEIN PURIFICATION KIT (BY SOLVENT FRACTIONATION)
The kit enables user to study the importance of solvent fractionation method for partial purification of protein of interest.
CAT. NO. BTK 130947
Contents
Protein sample
Fractionation Solutions
PAGE Reagents
User Manual
PREGNANCY SLIDE AGGLUTINATION TEST KIT
The kit enables user to study the test for pregnancy by slide agglutination method.
CAT. NO. BTK 130948
Contents
Anti-HCG Antibody
Latex Particles
Reaction Card
User Manual
ESTIMATION OF ACID PHOSPHATASE ACTIVITY IN SERUM KIT
Elevation of total acid phosphatase occurs in various bone liver diseases, Goucher’s disease & excessive destruction of platelets. The kit provides reagents for estimation of Acid Phosphatase level in serum by Alpha Naphthyl Phosphate Method.
CAT. NO. BTK 130949
Contents
Acid Phosphatase
Fast Red
Stabilizer Solution
User Manual
PROTEIN PURIFICATION KIT (BY SALT FRACTIONATION)
The kit enables user to study the importance of salt fractionation method for partial purification of protein.
CAT. NO. BTK 130950
Contents
Protein sample
Fractionation Solutions
PAGE Reagents
User Manual
ESTIMATION OF ALKALINE PHOSPHATASE ACTIVITY IN SERUM KIT
Serum ALP measurement is of particular interest in the Hepatobiliary disease and in bone diseases. The kit provides reagents for estimation of Alkaline Phosphatase level in serum by pNPP-AMP Method.
CAT. NO. BTK 130951
Contents
2-Amino-2-Methyl-1 Propanol Buffer
pNPP substrate
User Manual
BILIRUBIN ESTIMATION IN SERUM KIT
Bilirubin is helpful in measuring the severity of liver diseases. The kit provides reagents for estimation of bilirubin level in serum by Jendrassik and Grof Method
CAT. NO. BTK 130952
Contents
Sodium Nitrite
Sulphanilic Acid
Caffeine & Artificial Standard
User Manual
URIC ACID ESTIMATION IN SERUM KIT
The kit provides reagents for estimation of uric acid levels in serum by Enzymatic Method.
CAT. NO. BTK 130953
Contents
Buffer Regent
Enzyme Reagent
User Manual
CHOLESTEROL ESTIMATION IN SERUM KIT
The kit provides reagents for estimation of cholesterol levels in serum by Enzymatic CHOD-POD Method.
CAT. NO. BTK 130954
Contents
Cholesterol Regent
Cholesterol Standard
User Manual
TOTAL PROTEIN / ALBUMIN ESTIMATION IN SERUM KIT
The kit provides reagents for colorimetric estimation of total protein and albumin levels in serum.
CAT. NO. BTK 130955
Contents
BCG Reagent
Albumin Standard
Biuret Reagent
Protein Standard
User Manual
HDL & LDL ESTIMATION IN SERUM KIT
The kit provides reagents for estimation HDL and LDL levels in serum.
CAT. NO. BTK 130956
Contents
Precipitating Reagent
HDL Cholesterol Standard
Cholesterol Reagent
User Manual
TRIGLYCERIDES ESTIMATION IN SERUM KIT
The kit provides reagents for estimation triglycerides level in serum.
CAT. NO. BTK 130957
Contents
Triglycerides Reagent
Triglycerides Standard
User Manual
