Immunology Kits
DOT ELISA KIT
Dot ELISA is an immunological technique widely used for the qualitative detection of the antigen. The technique utilizes two antibodies, one attached to strip and other linked to enzyme. The presence of antigen in the test sample is indicated by color development in the form of dot on the strip.
CAT. NO.: BTK 140901
Contents
Dot ELISA Strips
Antibody-HRP Conjugates
TMB/H2O2
Test Serum Sample
Assay Buffer
User Manual
SANDWICH ELISA KIT
Sandwich ELISA technique utilizes two antibodies, one is immobilized to which antigen from the sample gets attached and other antibody linked to enzyme is then cross linked with antigen attached to first antibody. The antigen titre in the test sample is then detected by assaying the enzyme activity of second antibody.
CAT. NO.: BTK 140902
Contents
Standard Antigen / Test Antigen
Antibody
Antibody-HRP Conjugates
TMB/H2O2
Coating Buffer
Blocking Buffer
Microtitre Wells
User Manual
IMMUNOELECTROPHORESIS KIT
This technique is combination of both electrophoresis and diffusion. In the primary phase of the experiment a complex mixture of antigens is separated on the agarose gel by the technique of electrophoresis and in the second phase of the experiment antibodies are allowed to diffuse in the gel to form antigen-antibody precipitin lines.
CAT. NO.: BTK 140903
Contents
Antibodies
Antigen
Electrophoresis Buffer
Agarose
Glass Plate
Gel Punch
User Manual
RADIAL IMMUNODIFFUSION KIT
The technique is widely used to detect the antigen concentration in unknown sample. The technique is based on the principle that antigen diffuses radially in the gel containing uniformly distributed antibody forming a concentration gradient disk and when the antigen reaches an equivalence with the antibody concentration, a Ag-Ab precipitate forms which are opaque. The diameter of the ring is proportional to the concentration of antigen.
CAT. NO.: BTK 140904
Contents
Antiserum
Standard Antigens
Test Antigen
Assay Buffer
Glass Plate
Gel Punch
Template
User Manual
COUNTER CURRENT IMMUNOELECTROPHORESIS KIT
The technique is qualitative where the antigen and antibody diffusion in the gel is favored by process of electrophoresis.
CAT. NO.: BTK 140905
Contents
Antigen
Antibodies
Electrophoresis Buffer
Agarose
Gel Punch
Glass Plate
User Manual
ROCKET IMMUNOELECTROPHORESIS KIT
The technique is quantitative where the Ag-Ab complexes appear in the form of rockets. The height of rocket indicates the concentration of antigen. The Rocket immunoelectrophoresis technique utilizes both the electrophoresis and diffusion method.
CAT. NO.: BTK 140906
Contents
Antibodies
Standard Antigen
Test Antigen
Electrophoresis Buffer
Agarose
Glass Plate
Gel Punch
User Manual
OUCHTERLONY DOUBLE DIFFUSION KIT
(FOR ANTIGEN ANTIBODY PATTERNS)
In this technique the percent similarity between the antigens from various sources is examined on the basis of formation of various patterns of precipitin lines in the gel containing antiserum.
CAT. NO.: BTK 140907
Contents
Antigens
Antibodies
Agarose
Assay Buffer
Glass Plate
Gel Punch
Template
User Manual
OUCHTERLONY DOUBLE DIFFUSION KIT (FOR ANTIBODY TITRATION)
The technique is used to titre the antibodies from given sample.
CAT. NO.: BTK 140908
Contents
Antigens
Antibodies
Agarose
Assay Buffer
Glass Plate, Gel Punch & Template
User Manual
QUANTITATIVE PRECIPITIN ASSAY KIT
The technique is widely used to titre the antigen and antibody concentration in the given samples when both mixed in optimum proportions.
CAT. NO.: BTK 140909
Contents
Antigen
Antibodies
Assay Buffer
Glass Plate & Gel Punch
User Manual
ANTIBODY CAPTURE ELISA KIT
Antibody capture ELISA is immunological technique widely used for the quantitative assay of the antibody in the sample. The technique utilizes antigen immobilized to which antibody from the sample gets attached and anti-antibody linked to enzyme is then cross linked with antibody from sample. The antibody titre in the test sample is then detected by assaying the enzyme activity of secondary antibody
CAT. NO.: BTK 140910
Contents
Standard Antigen
Test Antigen
Antibody
Antibody-HRP Conjugates
TMB/H2O2
Coating Buffer
Blocking Buffer
Microtitre Wells
User Manual
ANTIGEN CAPTURE ELISA KIT
Antigen capture ELISA is immunological technique widely used for the quantitative assay of the antigen in the sample. The technique utilizes antibody immobilized to which antigen from the sample gets attached and second antibody linked to enzyme is then cross linked with antigen from sample attached to first antibody. The antigen titre in the test sample is then detected by assaying the enzyme activity of secondary antibody.
CAT. NO.: BTK 140911
Contents
Standard Antigen
Test Antigen
Antibody
Antibody-HRP Conjugates
TMB/H2O2
Coating Buffer
Blocking Buffer
Microtitre Wells
User Manual
WIDAL KIT (DETECTION OF Salmonella O AND H ANTIGEN)
The detection of Salmonella O and H antigen plays a major role in detection of the Salmonella infection in the patient and confirmation of Typhoid symptoms. The detection of these antigens can be achieved by reacting them with antibodie against them.
CAT. NO.: BTK 140912
Contents
Anti S. typhi ‘O’ Antibodies
Anti S. typhi ‘H’ Antibodies
Anti S. paratyphi ‘AH’ Antibodies
Anti S. paratyphi ‘BH’ Antibodies
User Manual
PREPARATION OF Salmonella O AND H ANTIGEN KIT
The kit enables user to prepare somatic and flagellar antigen of Salmonella.
CAT. NO.: BTK 140913
Contents
Stock culture
Nutrient Broth and Agar
Lysis Reagent
Purification Reagent
Titre Estimation Reagent
User Manual
VDRL (ELISA BASED) KIT
Syphillis is a sexually transmitted complex disease. The causative organism, Treponema pallidum cannot be grown on conventional laboratory culture media or in tissue culture. Infection is normally diagnosed by detecting antibodies specific for T. pallidum in the patient’s serum or CSF. The kit comprises of the reagents to perform and understand the syphilis test.
CAT. NO.: BTK 140914
Contents
Test Cells
Control Cells
Diluent
Positive Control
Negative Control
User Manual
COOMBS TEST KIT
The Coomb’s test is used to detect the presence of Rh antibodies in patient’s serum or red blood cells within the blood stream. Coombs’ test plays an important role in the field of diagnostics. It can be performed either as direct or indirect test. The kit enables user to perform both the type of tests and understand the principle of Coombs’ test.
CAT. NO.: BTK 140915
Contents
Coombs Serum
Anti-D Serum
Microcentrifuge Tubes
Glass Plates
User Manual
LATEX AGGLUTINATION KIT
Latex agglutination tests are very popular in clinical laboratories. These tests have been applied to detect over 100 infectious diseases and many other applications are currently available. The kit enables user to perform and interpret the functional and clinical aspect of the Latex agglutination test.
CAT. NO.: BTK 140916
Contents
Latex Beads
Glycine-Saline Buffer
Blocking Buffer
Antigen for Coating
Test Antiserum
Glass Plates
User Manual
OUDIN SINGLE DIFFUSION ASSAY KIT
It is a technique used to measure affinity of antibody towards antigen. An antigen and an antibody are held in an agar matrix in a test tube and allowed to diffuse towards one another. The kit enables user to perform and understand the test and its importance in immunological applications.
CAT. NO.: BTK 140917
Contents
Antigen
Antibodies
10X Assay Buffer
Agarose
Glass Tubes
User Manual
OAKLEY-FULTHORPE PROCEDURE KIT
In this test, antiserum incorporated into agarose is placed in a test-tube and then an antigen solution from the top and antibody from the bottom are allowed to diffuse to yield precipitation rings. The kit enables the user to perform and understand the test and its importance in immunological applications.
CAT. NO.: BTK 140918
Contents
Antigen
Antibodies
10X Assay Buffer
Agarose
Glass Tubes
User Manual
IMMUNOFLUORESCENCE KIT
Fluorescein is currently the most commonly-used fluorescent dye for FACS analysis. FITC is a small organic molecule, and is typically conjugated to proteins via primary amines. Fluorescein is typically excited by the 488 nm line of an argon laser, and emission is collected at 530 nm. The kit enables user to perform the fluorescence test for immunological reaction.
CAT. NO.: BTK 140919
Contents
Antibody for Coupling
Conjugation Buffer
Gel Filtration Matrix
10X PBS
Fluorescein Isothiocyanate
Ammonium Chloride
Column
User Manual
IMMUNOGLOBULIN PURIFICATION KIT (FROM HUMAN SERUM)
When human serum is added to an ion exchange column, most of the serum proteins become bound to the column. However, by using a buffer with selective pH range, IgG is not absorbed and is eluted in the first fractions. The kit provides reagents not only to purify IgG from the serum but also assess its purity.
CAT. NO.: BTK 140920
Contents
Tris-HCl Buffer
Buffered Saline
Sephadex
Dialysis Tubes
10X Electrophoresis Solution
Cellulose Acetate Paper Strips
Column
Glass Wool
User Manual
IMMUNOPRECIPITATION KIT
In this Immunological testing, antigens are mixed with the antiserum and the precipitation is measured using UV spectrophotometer to analyze the amount and intensity of complexes formed by the immune reaction.
CAT. NO.: BTK 140921
Contents
Antigen
Antiserum
Buffered Saline
Microcentrifuge Tubes
Graph Paper Sheets
User Manual
CHICKEN IMMUNOGLOBULIN PURIFICATION KIT
The kit enables user to perform separation and purification of IgY from the egg yolk and to assess its purity.
CAT. NO.: BTK 140922
Contents
Phosphate Buffer
Storage Phosphate Buffer
Polyethylene Glycol
User Manual
BLOOD GROUPING KIT
The kit provides solutions sufficient to perform the tube and slide test for identification of blood group and Rh factor.
CAT. NO.: BTK 140923
Contents
Anti-A, Anti-B, Anti-D (Rho)
Saline
Mixing Sticks
Glass Plates
Dispensers
User Manual
