Molecular Biology Kits

NUCLEIC ACID GEL ELECTROPHORESIS KIT

The kit enables the practical handling and interpretation of electrophoretic separation of nucleic acids (DNA and RNA) on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120901

Contents
Nucleic Acid Samples (DNA & RNA)
50X TAE
Gel Loading Dye
Staining Dye
Agarose
Semilog Graph Paper Sheets
User Manual

DNA MOLECULAR SIZE DETERMINATION KIT

The kit describes the practical handling and interpretation of electrophoretic separation of DNA on agarose gel in submarine gel electrophoresis system and determination of its molecular weight.

CAT. NO. BTK 120902

Contents
DNA
Molecular Weight Marker Lane
50X TAE
Gel Loading Dye
Staining Dye
Agarose
Semilog Graph Paper Sheets
User Manual

NATIVE RNA GEL ELECTROPHORESIS KIT

The kit enables the practical handling and interpretation of electrophoretic separation of RNA in its native form on agarose gel in submarine gel electrophoresis system and determination of its native molecular weight.

CAT. NO. BTK 120903

Contents
RNA
50X TAE
Gel Loading Dye
Staining Dye
Agarose
Semilog Graph Paper Sheets
User Manual

MOPS RNA GEL ELECTROPHORESIS KIT

The kit describes the practical handling and interpretation of electrophoretic separation of RNA under denatured conditions on agarose gel in submarine gel electrophoresis system and determination of its molecular weight.

CAT. NO. BTK 120904

Contents
RNA
10X MOPS Buffer
Formaldehyde
Gel Loading Dye
Staining Dye
Agarose
User Manual

DNA ESTIMATION KIT

The kit describes practical performance and comparison of two widely used techniques for estimation of DNA. The techniques are colourimetric DPA method and UV spectrophotometric absorbance method.

CAT. NO. BTK 120905

Contents
DNA Standard & DNA Sample
DPA Reagent
Nomograph
Graph Paper Sheets
User Manual

DNA DOT QUANTITATION KIT

The kit describes practical performance of technique for estimation of DNA by comparing the fluorescence of ethidium bromide stained test DNA with that of the fluorescence of ethidium bromide stained various concentrations of standard DNA.

CAT. NO. BTK 120906

Contents
DNA Standards
DNA Sample
Ethidium Bromide
User Manual

RNA ESTIMATION KIT

The kit describes practical performance of estimation of RNA by colourimetric Orcinol method and UV spectrophotometric absorbance method.

CAT. NO. BTK 120907

Contents
RNA Standard
RNA Sample
Orcinol Reagent
Graph Paper Sheets
User Manual

GENOMIC DNA EXTRACTION KIT (FROM BACTERIA)

The kit enables the user practical handling of extraction of genomic DNA from bacterial cells and detection of presence of DNA in the extract by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120908

Contents
Bacterial Stock
Cell Lysis Buffer
Phenol / Chloroform
TE Buffer
Sodium Acetate
Isopropanol
Agarose Gel Electrophoresis Contents
LB Broth and Agar
User Manual

PLANT DNA EXTRACTION KIT

The kit describes the practical handling and interpretation of extraction of DNA from plant cells and detection of extracted DNA by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120909

Contents
2-Mercaptoethanol
CTAB Extraction Solution
CTAB / NaCl Solution
CTAB Precipitation Solution
High-salt TE Buffer
Isoporopanol
Chloroform / Isoamyl alcohol
Agarose Gel Electrophoresis Contents
User Manual

YEAST DNA EXTRACTION KIT

The kit describes the practical handling and interprutation of extraction of DNA from yeast cells and detection of it’s presence by electrophoresis on agaros gel in submarine gel electrophoresis system.

CAT. NO. BTK 120910

Contents
Stock Culture
Cell Lysis Buffer
Phenol / Chloroform / Isoamyl alcohol
TE Buffer
RNase A
Ammonium Acetate Solution
Agarose Gel Electrophoresis Contents
Universal Yeast Broth and Agar
User Manual

FUNGAL DNA EXTRACTION KIT

The kit describes the practical handling and interpretation of extraction of DNA from fungal mycelia and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120911

Contents
Stock Culture
Sand
Extraction Buffer
TE Buffer
Phenol / Chloroform / Isoamyl Alcohol
CTAB Solution
Agarose Gel Electrophoresis Contents
Potato Dextrose Broth and Agar
User Manual

DNA EXTRACTION KIT (FROM MAMMALIAN TISSUES)

The kit describes the practical handling and interpretation of extraction of DNA from mammalian cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120912

Contents
Digestion Buffer
Phosphate Buffered Saline
Phenol / Chloroform / Isoamyl alcohol
Ammonium Acetate
Ethanol
TE Buffer
Agarose Gel Electrophoresis Contents
User Manual

DNA EXTRACTION KIT (FROM BLOOD CELLS)

The kit describes the practical handling and interpretation of extraction of DNA from blood cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120913

Contents
Phosphate Buffered Saline
Cell Lysis Buffer
Ethanol
TE Buffer
Agarose Gel Electrophoresis Contents
User Manual

DNA EXTRACTION KIT (FROM BACTERIOPHAGES)

The kit describes the practical handling and interpretation of extraction of DNA from bacteriophage lysate and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120914

Contents
Phosphate Buffered Saline
Cell Lysis Buffer
Ethanol
TE Buffer
Agarose Gel Electrophoresis Contents
User Manual

RNA EXTRACTION KIT (FROM BACTERIA)

The kit describes the practical handling and interpretation of extraction of total RNA from bacterial cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120915

Contents
Bacterial Stock
Cell Lysis Buffer
Protoplasting Buffer
Lysozyme Saturated NaCl Solution
Agarose Gel Electrophoresis Contents
LB Broth and Agar
User Manual

RNA EXTRACTION KIT (FROM PLANT CELLS)

The kit describes the practical handling and interpretation of extraction of total RNA from plant cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120916

Contents
Grinding Buffer
Phenol Solution
Chloroform
Lithium Chloride Solution
Sodium Acetate Solution
Ethanol
Agarose Gel Electrophoresis Contents
User Manual

RNA EXTRACTION KIT (FROM YEAST)

The kit describes the practical handling and interpretation of extraction of total RNA from yeast cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120917

Contents
Stock Culture
Cell Lysis Buffer
Phenol / Chloroform / Isoamyl alcohol
TE Buffer
Ammonium Acetate Solution
Agarose Gel Electrophoresis Contents
Universal Yeast Broth and Agar
User Manual

RNA EXTRACTION KIT (FROM MAMMALIAN TISSUES)

The kit describes the practical handling and interpretation of extraction of total RNA from mammalian cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120918

Contents
Digestion Buffer
Phosphate Buffered Saline
Phenol / Chloroform / Isoamyl alcohol
Ammonium Acetate
TE Buffer
Agarose Gel Electrophoresis Contents
User Manual

mRNA PREPARATION KIT (FROM MAMMALIAN TISSUES)

The kit describes the practical handling and interpretation of extraction of mRNA from mammalian cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120919

Contents
Denaturation Solution
Phenol
Tris Buffer
Wash Buffer
NaCl Solution
High Affinity Spin Columns
User Manual

RESTRICTION DIGESTION KIT

The kit describes the practical performance of one of the most vital steps used in recombinant DNA technology i.e. digestion of DNA by restriction endonucleases.

CAT. NO. BTK 120920

Contents
DNA
Hind III & EcoRI
Activity Assay Buffer
Control Restricted DNA
Agarose Gel Electrophoresis Contents
User Manual

RESTRICTION DIGESTION MAPPING KIT

The kit describes the practical handling and interpretation of single and double digestion of DNA by restriction endonucleases, detection of digest by electrophoresis on agarose gel in submarine gel electrophoresis system and mapping of restricted DNA for detection of presence of numbers of endonuclease sites and their order of presence on DNA.

CAT. NO. BTK 120921

Contents
DNA
Endonucleases I
Endonucleases II
Activity Assay Buffer
Control Restricted DNA
Agarose Gel Electrophoresis Contents
User Manual

LIGATION KIT

The kit describes the practical performance of one of the most vital steps used in recombinant DNA technology i.e. joining of cut DNA fragments by Ligase and detection of ligated DNA fragments by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120922

Contents
DNA
Ligase
Ligase Activity Assay Buffer
Nuclease Free Water
Agarose Gel Electrophoresis Contents
User Manual

PLASMID DNA PREPARATION KIT

The kit describes the practical handling and interpretation of plasmid DNA extraction from bacterial cells and detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120923

Contents
Stock Culture
RNase
Ampicillin
Plasmid Extraction Solution I, II, III & IV
Agarose Gel Electrophoresis Contents
LB Broth and Agar
User Manual

PLASMID DNA CLONING KIT

The kit describes basic understanding and practical techniques used in the recombinant DNA technology by cloning of foreign DNA fragment in plasmid DNA through restriction and ligation, transfer of recombinant DNA in suitable host by the technique of artificial transformation and detection of expression of cloned DNA in transformed cells.

CAT. NO. BTK 120924

Contents
Host Culture
Restriction Enzyme & Assay Buffer
Ligase & Assay Buffer
Transformation solution
LB Broth and Agar
Cloned Plasmid DNA Detection Markers
User Manual

DNA SILVER STAINING Kit

The kit describes the practical handling of most sensitive staining method available for staining of DNA from Polyacrylamide Gel with silver staining.

CAT. NO. BTK 120925

Contents
Fixing Solution
Destaining Solution
Silver Nitrate Solution
Developing Solution
PAGE Contents
User Manual

DNA Tm ESTIMATION KIT

The kit describes the practical handling and interpretation of estimation of Tm of DNA by spectrophotometric analysis and detection of denatured DNA by Electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120926

Contents
DNA
Reaction Buffer
Agarose Gel Electrophoresis Contents
Nomograph
User Manual

ENDODEOXYRIBONUCLEASE CHROMATIN DIGESTION KIT

The kit describes the practical handling and interpretation of action of endodeoxyribonuclease on DNA and detection of its action by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120927

Contents
DNA
Endodeoxyribonuclease
Assay Buffer
Agarose Gel Electrophoresis Contents
User Manual

PCR KIT

The kit describes the practical handling and interpretation of basic PCR technique and detection of its products by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120928

Contents
Template DNA
10mM dNTP Mix
Forward Primer
Reverse Primer
Taq DNA Polymerase & Assay Buffer
Agarose Gel Electrophoresis Contents
PCR Tubes
User Manual

REPORTER GENE ASSAY KIT

Reporter genes are used to determine if the gene of interest has been taken up or expressed in the cell. The kit allows for expression of gene in bacterial cell Under induced state and detection of protein product of expressed gene by cell lysis and performing SDS-PAGE for lysed bacterial extract on Vertical Gel Electrophoresis System.

CAT. NO. BTK 120929

Contents
Culture Stock
Antibiotic
Inducer
Cell Lysis Buffer
Protein Marker Lane
SDS PAGE Contents
LB Broth and Agar
User Manual

BACTERIAL GENE EXPRESSION KIT

The kit enables the practical approach for expression of gene in bacterial cell under induced state and detection of protein product of expressed gene by cell lysis and performing SDSPAGE for lysed bacterial extract on Vertical Gel Electrophoresis System.

CAT. NO. BTK 120930

Contents
Culture Stock
Ampicillin & IPTG
Cell Lysis Buffer
Protein Marker Lane
SDS PAGE Contents
LB Broth and Agar
User Manual

STUDY OF Agrobacterium MEDIATED TRANSFORMATION KIT

The kit enables user to practically infect tissue cultured tobacco plantlet with Agrobacterium containing gus expression vector and expression of gus gene by transformed tobacco plantlet.

CAT. NO. BTK 120931

Contents
Culture Stock
Acetosyringoin
Glucose + Fructose Solution
X-Gluc
Cefotaxamine
YEP Broth and Agar
MR Macro and Micro Nutrients
User Manual

GFP CLONING KIT

The kit describes the practical handling and interpretation of cloning of Green Fluorescent Protein Gene in suitable vector and artificial transformation and expression of cloned GFP gene in suitable host.

CAT. NO. BTK 120932

Contents
Culture Stock
Sterile Calcium Chloride Solution
Control DNA
Ampicillin
T4 DNA Ligase and Assay Buffer
Insert and Vector
LB Broth and Agar
User Manual

NORTHERN BLOTTING KIT

The kit describes the practical handling and interpretation of fractionation of RNA by electrophoresis and then transfer of the separated patterns of RNA on solid support like nylon membrane.

CAT. NO. BTK 120933

Contents
RNA
Molecular Weight Marker Lane
MOPS RNA Gel Electrophoresis Contents
Nylon Memebrane
Denaturation & Neutralization Solution
20X and 2X SSC
Whatman paper, Paper Towels,
Glass Plate, Sponge, Plastic Wrap
User Manual

RFLP DEMONSTRATION KIT

The kit enables user the practical understanding and implication of the most renewed technique of molecular biology. RFLP or in general DNA fingerprinting technique is widely utilized to evaluate the molecular differences between various samples.

CAT. NO. BTK 120934

Contents
DNA Marker Lane
Test DNA
Control DNA
Restriction Enzyme
Assay Buffer
Agarose Gel Electrophoresis Contents
User Manual

PREPARATION OF COMPETENT CELL SYSTEM KIT

The kit provides solution for the one step preparation of competent cells ready for transformation protocol.

CAT. NO. BTK 120935

Contents
Competent Cell Preparation Solution
User Manual

GENOTYPE ANALYSIS KIT

The kit describes the practical handling and interpretation of PCR amplification and agarose gel electrophoresis of the sample DNA and test DNA and to test the molecular homogeneity of the two.

CAT. NO. BTK 120936

Contents
Test DNA & Sample DNA
Taq DNA Polymerase
dNTP Mix
Primer Set
Assay Buffer
Agarose Gel Electrophoresis Contents
PCR Tubes
User Manual

FUELGEN CHROMATIN STAINING KIT

The kit describes the practical handling and interpretation of staining of chromatin by Fuelgen method.

CAT. NO. BTK 120937

Contents
Basic Fuchsin Stain
Fast Green Stain
Acetocarmine Solution
Acetoorcein Solution
Sodium Metabisulphide Solution
Potassium Metabisulphide Solution
User Manual

TRANSFORMATION KIT (IN BACTERIA)

The kit provides the practical understanding and handling of preparation of competent cells, artificial transformation of competent cells with plasmid DNA,detection of transformed cells and estimation of transformation frequency.

CAT. NO. BTK 120938

Contents
Culture Stock
X-Gal
IPTG
Plasmid DNA
Sterile Calcium Chloride Solution
Ampicillin
LB Broth and Agar
User Manual

CONJUGATION KIT (IN BACTERIA)

The kit describes the practical handling and interpretation of transfer of DNA from Hfr strain (High Frequency Recombinant Bacterial Donor) to Recipient strain and detection of Recipient strains for transferred DNA.

CAT. NO. BTK 120939

Contents
Donor Strain
Recepient Strain
Streptomycin
Tetracycline
LB Broth
Agar
User Manual

PROTOPLAST FUSION KIT (IN BACTERIA)

The kit describes the practical handling and interpretation of preparation of protoplasts of two different auxotrophic bacterial strains, their fusion and regeneration to obtain recombinant prototrophic strain.

CAT. NO. BTK 120940

Contents
Stock Culture A & Stock Culture B
PEG Buffer
Protoplasting Fusion Buffer A and B
Regeneration Buffer and Medium
Protoplast Detection Marker
LB Broth and Agar
User Manual

PLANT PROTOPLAST PREPARATION KIT

The kit describes the practical handling and interpretation for the preparation of protoplasts from plant source.

CAT. NO. BTK 120941

Contents
Protoplasting Enzyme solution
User Manual

COMET ASSAY KIT

The Comet Assay or single cell gel electrophoresis (SCGE) assay is a rapid, sensitive and relatively simple method for detecting DNA damage at the level of individual cells. It combines the simplicity of biochemical techniques for detecting DNA single strand breaks, with the single cell approach typical of cytogenetics assays.

CAT. NO. BTK 120942

Contents
Lysis, Alkaline & Neutralization Solution
Electrophoresis Buffer
Hydrogen Peroxide
Triton X-100
Agarose
Low Melting Point Agarose
Comet Plates
User Manual

SOUTHERN BLOTTING KIT (BY ELECTRO-TRANSFER)

The kit enables the user to carry out fractionation of DNA by electrophoresis & then electroblot to transfer the separated patterns of DNA on solid support like nylon membrane.

CAT. NO. BTK 120943

Contents
DNA Sample
Agarose Gel Electrophoresis Contents
Ethidium Bromide
Electrotransfer Buffer
Nylon Membrane
Filter Papers
User Manual

SOUTHERN BLOTTING KIT (BY CAPILLARY-TRANSFER)

The kit enables the user to carry out fractionation of DNA by electrophoresis & then capillary transfer of the separated patterns of DNA on solid support like nylon membrane.

CAT. NO. BTK 120944

Contents
DNA Sample
Agarose Gel Electrophoresis Contents
Ethidium Bromide
Denaturation ant Neutralization Solution
SSC Solution
Nylon Membrane
Filter Papers; Paper Towels; Glass Plate;
Sponge etc.
User Manual

PREPARATION OF ssDNA TEMPLATE KIT

The kit describes the practical handling and interpretation of action of exonucleases on dsDNA so as to form ssDNA template and detection of formed ssDNA by electrophoresis on agarose gel in submarine gel electrophoresis.

CAT. NO. BTK 120945

Contents
dsDNA
Exonuclease (5’ 3’)
Assay Buffer
Reaction Termination Buffer
Agarose Gel Electrophresis Contents
User Manual

AGAROSE GEL ELECTROPHORESIS KIT

The kit enables the user to carry out fractionation of DNA by electrophoresis using agarose as the gel matrix.

CAT. NO. BTK 120946

Contents
DNA Sample
Agarose Gel Electrophoresis Contents
DNA Staining Dye
User Manual

RAPID PLASMID DNA PREPARATION KIT

The kit enables the user to carry out extraction of plasmid DNA by boiling miniprep. This method is recommended for preparing small amounts of plasmid DNA from 1 to 24 cultures. The method is extremely quick. The kit also enables user to carry out electrophoretic analysis of the extracted plasmid DNA.

CAT. NO. BTK 120947

Contents
Host Culture
Lysozyme
STET Solution
RNase
Agarose Gel Electrophoresis Contents
DNA Staining Dye
User Manual

MITOCHONDRIAL DNA ISOLATION KIT (FROM PLANT)

Although DNA in the nuclear chromosome and the cytoplasmic mitochondria of a cell are composed of complementary polynucleotide chains, their numbers, sizes, and geometric arrangements are quite different. The kit enables user to extract the mitochondrial DNA from plant.

CAT. NO. BTK 120948

Contents
Isolation Medium
Resuspension Medium
Extraction Solution I, II and III
Agarose Gel Electrophoresis Contents
DNA Staining Dye
User Manual

TRANSPOSONS DETECTION KIT (FROM BACTERIA)

Transposons or jumping genes or mobile genetic elements present in bacteria are known for their shifting of positions within the DNA by the process termed as ‘Transposition’. The kit enables user to study the process of transposition through its contents.

CAT. NO. BTK 120949

Contents
Culture Strain A
Culture Strain B
Marker components
LB Broth
Agar
User Manual

TRANSDUCTION KIT (IN BACTERIA)

The transfer of the genetic material from one bacterium to other bacteria can take place by three most important ways viz., conjugation, transformation and transduction. In transduction the transfer of genetic material takes place by the mediator which is a bacteriophage. The lysogenic bacteriophages are responsible for the process of Transduction. The kit enables user to study the process of transduction through its contents.

CAT. NO. BTK 120950

Contents
Culture Strain A
Culture Strain B
Bactriophage Strain
Marker components
LB Broth
Agar
User Manual

PLASMID CURING KIT (FROM BACTERIA)

Plasmids are the extra chromosomal material and are inherited by the progeny cells. The curing of plasmid means the elimination of plasmid from the bacterial cells harboring it. The process of curing takes place naturally due to incompatibility of the plasmid in a bacterial cell. Artificially curing can be induced using various physical and chemical agents. The kit enables user to study the process of artificial curing of plasmid through its contents.

CAT. NO. BTK 120951

Contents
Culture Strain
Curing Agent
Marker components
LB Broth
Agar
User Manual

PREPARATION OF BACTERIAL PROTOPLASTS KIT

Preparation of Bacterial Protoplasts Kit. The kit enables user to prepare protoplasts from Gram positive and Gram negative bacterial cells for numerous molecular and recombinant studies.

CAT. NO. BTK 120952

Contents
Bacterial Culture
Lysozyme
PEG Buffer
Protoplast Preparation Buffer A
Protoplast Preparation Buffer B
LB Broth and Agar
User Manual

EXTRACTION OF DNA FROM GEL KIT

Separation and characterization of DNA using agarose gel electrophoresis is one of the most widely used methods. The separated DNA band on the gel can be reformed to liquid form by many methods. This kit is based on silica method.

CAT. NO. BTK 120953

Contents
DNA
Agarose Gel Electrophoresis Contents
Silica
Extraction Buffer
Gel Cutter
User Manual

PLASMID DNA RESTRICTION KIT

The kit enables user to perform the digestion of Plasmid DNA with Endonucleases and detection of digested Plasmid DNA fragments by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120954

Contents
Plasmid DNA
Endonuclease
Endonuclease Assay Buffer
Agarose Gel Electrophoresis Contents
User Manual

PLASMID DNA LIGATION KIT

The kit enables the user to perform one of the most vital steps used in recombinant DNA technology that is joining of cut plasmic DNA by ligase and detection of ligated plasmid DNA by electrophoresis on agarose gel in submarine gel electrophoresis system.

CAT. NO. BTK 120955

Contents
Plasmid DNA
Ligase
Ligase Assay Buffer
Agarose Gel Electrophoresis Contents
User Manual

DNA EXTRACTION KIT (FROM CULTURED MAMMALIAN CELLS)

Extraction of DNA from cultured mammalian cells & detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system can be performed using this kit.

CAT. NO. BTK 120956

Contents
Extraction Buffer
Phenol / Chloroform / Isoamyl Alcohol
Ammonium Acetate
TE Buffer
Agarose Gel Electrophoresis Contents
User Manual

DNA EXTRACTION KIT (FROM PLANT SUSPENSION CELLS)

Extraction of DNA from Plant suspension cultures & detection of its presence by electrophoresis on agarose gel in submarine gel electrophoresis system can be performed using this kit.</>

CAT. NO. BTK 120957

Contents
Extraction Buffer
Precipitation Buffer
High Salt TE Buffer
Chloroform / Isoamyl alcohol
Agarose Gel Electrophoresis Contents
User Manual

MITOCHONDRIAL DNA EXTRACTION KIT (FROM MAMMALIAN TISSUES)

The kit demonstrates extraction of mitochondria and preparation of DNA from extracted mitochondria. The isolated DNA is detected for Its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120958

Contents
Lysis Buffer
Extraction Buffer
DNase Lysis Buffer
Gradient Solutions
TE Buffer
Agarose Gel Electrophoresis Contents
User Manual

CHLOROPLAST DNA EXTRACTION KIT (FROM PLANT)

The kit demonstrates extraction of mitochondria and preparation of DNA from extracted mitochondria. The isolated DNA is detected for its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120959

Contents
Homogenization Buffer
Lysis Buffer
DNase Lysis Buffer
Extraction Solutions
Agarose Gel Electrophoresis Contents
User Manual

MITOCHONDRIAL RNA EXTRACTION KIT (FROM PLANT)

The kit demonstrates extraction of mitochondria and preparation of RNA from extracted mitochondria. The isolated RNA is detected for its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120960

Contents
Extraction Buffer
Lysis Buffer
Isolation Buffer
Gradient Solutions
Wash Buffer
Agarose Gel Electrophoresis Contents
User Manual

MITOCHONDRIAL RNA EXTRACTION KIT (FROM MAMMALIAN TISSUES)

The kit demonstrates extraction of mitochondria and preparation of RNA from extracted mitochondria. The isolated RNA is detected for its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120961

Contents
Extraction Buffer
Precipitation Buffer
Wash Buffer
High Salt Solution
Agarose Gel Electrophoresis Contents
User Manual

CHLOROPLAST RNA EXTRACTION KIT (FROM PLANT)

The kit demonstrates extraction of chloroplast and preparation of RNA from extracted Chloroplasts. The isolated RNA is detected for its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120962

Contents
Extraction Buffer
Homogenization Buffer
Lysis Buffer
Precipitation Buffer
Agarose Gel Electrophoresis Contents
User Manual

mRNA EXTRACTION KIT (FROM PLANT)

The kit demonstrates purification of mRNA from the total RNA extracted from plant material. The isolated mRNA is detected for its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120963

Contents
Grinding Buffer
Lithium Chloride
Binding Buffer
Wash Buffer
Elution Buffer
TLE Equilibrated Phenol
Agarose Gel Electrophoresis Contents
User Manual

RNA EXTRACTION KIT (FROM FUNGI)

The kit demonstrates extraction of total RNA and its detection by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120964

Contents
Stock Culture
Extraction Buffer
Agarose Gel Electrophoresis Contents
Potato Dextrose Broth and Agar
User Manual

RNA EXTRACTION KIT (FROM BLOOD)

The kit demonstrates extraction of total RNA and its detection by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120965

Contents
Extraction Buffer
Phosphate Buffer Saline
High Salt Solution
Agarose Gel Electrophoresis Contents
User Manual

TRILYS EXTRACTION OF RNA KIT

The kit demonstrates extraction of RNA by Trilys solution and its detection by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120966

Contents
Trilys Solution
Chloroform / Isoamyl alcohol
Isopropanol
Agarose Gel Electrophoresis Contents
User Manual

mRNA PREPARATION KIT (FROM BLOOD)

The kit demonstrates purification of mRNA from the total RNA extracted from blood. The isolated mRNA is detected for its presence by electrophoresis on agarose gel in submarine gel electrophoresis system using this kit.

CAT. NO. BTK 120967

Contents
Lysis Buffer
Binding Buffer
Wash Buffer
Elution Buffer
Agarose Gel Electrophoresis Contents
User Manual


mRNA PREPARATION KIT (FROM YEAST)

The kit demonstrates purification of mRNA from the total RNA
extracted from yeast cells. The isolated mRNA is detected for
its presence by electrophoresis on agarose gel in submarine
gel electrophoresis system using this kit.

CAT. NO. BTK 120968

Contents
Culture Stock
Lysis Buffer
Binding Buffer
Wash Buffer
Elution Buffer
Agarose Gel Electrophoresis Contents
User Manual

RESTRICTION MODIFICATION KIT

The Bacterial Defense system against invading bacteriophages includes production of Endonucleases which cleaves the bacteriophage DNA. Bacterial cells protect its own DNA against the action of Endonucleases by methylating the Endonuclease sites present on DNA with the help of enzyme methylase. This system is called as Restriction Modification system. The kit demonstrates the impact of methylase enzyme on endonuclease activity for a particular sample of DNA.

CAT. NO. BTK 120969

Contents
Restriction Enzymes
Methylase
Activity Assay Buffers
Agarose Gel Electrophoresis Contents
User Manual